Recombinant Protein leading To Cardiac Pressure

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Glycolaldehyde-Modified proteins trigger adversarial practical and structural aortic reworking resulting in cardiac strain overload

Rising proof helps the position of superior glycation finish merchandise (AGEs) within the growth of diabetic vascular problems and cardiovascular illnesses (CVDs). We now have proven that high-molecular-weight AGEs (HMW-AGEs), current in our Western eating regimen, impair cardiac perform. Whether or not HMW-AGEs have an effect on vascular perform stays unknown.

On this research, we aimed to research the affect of continual HMW-AGEs publicity on vascular perform and construction. Grownup male Sprague Dawley rats had been each day injected with HMW-AGEs or management resolution for six weeks. HMW-AGEs animals confirmed intracardiac strain overload, characterised by elevated systolic and imply pressures.

The contraction response to PE was elevated in aortic rings from the HMW-AGEs group. Leisure in response to ACh, however not SNP, was impaired by HMW-AGEs. This was related to decreased plasma cyclic GMP ranges. SOD restored ACh-induced leisure of HMW-AGEs animals to manage ranges, accompanied by a decreased half-maximal efficient dose (EC50).

 

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Lastly, collagen deposition and intima-media thickness of the aortic vessel wall had been elevated with HMW-AGEs. Our knowledge show that continual HMW-AGEs publicity causes adversarial vascular remodelling. That is characterised by disturbed vasomotor perform attributable to elevated oxidative stress and structural modifications within the aorta, suggesting an vital contribution of HMW-AGEs within the growth of CVDs.

 

PRDM1 Recombinant Protein (Human)

Giant-scale, unbiased proteomics research are constrained by the complexity of the plasma proteome. Right here we report a extremely parallel protein quantitation platform integrating nanoparticle (NP) protein coronas with liquid chromatography-mass spectrometry for environment friendly proteomic profiling. A protein corona is a protein layer adsorbed onto NPs upon contact with biofluids.

Various the physicochemical properties of engineered NPs interprets to distinct protein corona patterns enabling differential and reproducible interrogation of organic samples, together with deep sampling of the plasma proteome. Spike experiments verify a linear sign response.

The median coefficient of variation was 22%. We screened 43 NPs and chosen a panel of 5, which detect greater than 2,000 proteins from 141 plasma samples utilizing a 96-well automated workflow in a pilot non-small cell lung most cancers classification research.

Our streamlined workflow combines depth of protection and throughput with exact quantification based mostly on distinctive interactions between proteins and NPs engineered for deep and scalable quantitative proteomic research.

 

Polyclonal BLIMP1 / PRDM1 Antibody (Inside)

Description: A polyclonal antibody raised in Goat that acknowledges and binds to Human BLIMP1 / PRDM1 (Inside). This antibody is examined and confirmed to work within the following purposes:

 

 

    • Bcl-2 inhibitors show an efficient exercise in acute myeloid leukemia (AML), however its scientific efficacy as a monotherapy was restricted partially owing to failure to focus on different antiapoptotic Bcl-2 household proteins, akin to Mcl-1.

 

    • On this context, the mixture technique could also be a promising strategy to beat this barrier. Right here, we report the preclinical efficacy of a novel technique combining ABT-199 with triptolide (TPL), a pure product extracted from a standard Chinese language medication, in AML.

 

    • Mixture therapy exhibited markedly elevated cytotoxicity in leukemic cells regardless of p53 standing whereas largely sparing regular cells of the hematopoietic lineage. Furthermore, co-administration of ABT-199 with TPL dramatically suppressed leukemia development in addition to extended animal survival in a xenograft AML mannequin.

 

    • The potentiated impact of ABT-199 and TPL towards AML was related to activation of the mitochondrum-related intrinsic apoptotic pathway by a mechanism reciprocally modulating Bcl-2 household proteins. On this case, TPL not solely downregulated Mcl-1 but additionally upregulated proapoptotic BH3-only proteins, thereby overcoming the resistance towards ABT-199.

 

    • Conversely, ABT-199 abrogated Bcl-2-mediated cytoprotection towards TPL. Collectively, these findings recommend that the routine combining TPL and ABT-199 may be energetic towards AML by inducing sturdy apoptosis by reciprocal regulation of anti- and proapoptotic Bcl-2 household proteins, due to this fact offering a powerful rationale for the scientific investigation of this mixture routine for the therapy of AML.

 

 

PRDM1 Antibody

CSB-PA193485-
EUR 335
Description: A polyclonal antibody against PRDM1. Recognizes PRDM1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:1000

PRDM1 Antibody

CSB-PA193485-100ul 100ul
EUR 316
Description: A polyclonal antibody against PRDM1. Recognizes PRDM1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:1000

PRDM1 siRNA

20-abx929665
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

PRDM1 siRNA

20-abx929666
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

PRDM1 Antibody

48403-100ul 100ul
EUR 333

PRDM1 Antibody

48403-50ul 50ul
EUR 239

PRDM1 Blocking Peptide

BF0254-BP 1mg
EUR 195

anti-PRDM1/Blimp1

YF-PA10478 50 ul
EUR 363
Description: Mouse polyclonal to PRDM1/Blimp1

anti-PRDM1/Blimp1

YF-PA10479 100 ul
EUR 403
Description: Rabbit polyclonal to PRDM1/Blimp1

anti-PRDM1/Blimp1

YF-PA10480 100 ug
EUR 403
Description: Rabbit polyclonal to PRDM1/Blimp1

anti-PRDM1/Blimp1

YF-PA10481 100 ug
EUR 403
Description: Rabbit polyclonal to PRDM1/Blimp1

anti-PRDM1/Blimp1

YF-PA23301 50 ul
EUR 334
Description: Mouse polyclonal to PRDM1/Blimp1

anti-PRDM1 (5E7)

LF-MA30366 100 ul
EUR 486
Description: Mouse Monoclonal to PRDM1

PRDM1 cloning plasmid

CSB-CL018638HU-10ug 10ug
EUR 474
Description: A cloning plasmid for the PRDM1 gene.

PRDM1 Polyclonal Antibody

A-2001 100 µl Ask for price
Description: kits suitable for this type of research

PRDM1 Rabbit pAb

A1960-100ul 100 ul
EUR 308

PRDM1 Rabbit pAb

A1960-200ul 200 ul
EUR 459

PRDM1 Rabbit pAb

A1960-20ul 20 ul
EUR 183

PRDM1 Rabbit pAb

A1960-50ul 50 ul
EUR 223

PRDM1 Conjugated Antibody

C48403 100ul
EUR 397

Anti-PRDM1 antibody

STJ116165 100 µl
EUR 277
Description: This gene encodes a protein that acts as a repressor of beta-interferon gene expression. The protein binds specifically to the PRDI (positive regulatory domain I element) of the beta-IFN gene promoter. Transcription of this gene increases upon virus induction. Two alternatively spliced transcript variants that encode different isoforms have been reported.

PRDM1 ELISA KIT|Human

EF005444 96 Tests
EUR 689

Anti-PRDM1/Blimp1 (2B10)

YF-MA12126 100 ug
EUR 363
Description: Mouse monoclonal to PRDM1/Blimp1

Anti-PRDM1/Blimp1 (4A6)

YF-MA12127 100 ug
EUR 363
Description: Mouse monoclonal to PRDM1/Blimp1

Anti-PRDM1/Blimp1 (1G6)

YF-MA12128 100 ug
EUR 363
Description: Mouse monoclonal to PRDM1/Blimp1

Anti-PRDM1/Blimp1 (2F8)

YF-MA12129 100 ug
EUR 363
Description: Mouse monoclonal to PRDM1/Blimp1

Anti-PRDM1/Blimp1 (1F2)

YF-MA12130 100 ug
EUR 363
Description: Mouse monoclonal to PRDM1/Blimp1

Anti-PRDM1/Blimp1 (2A3)

YF-MA12131 100 ug
EUR 363
Description: Mouse monoclonal to PRDM1/Blimp1

Anti-PRDM1/Blimp1 (1G9)

YF-MA12132 100 ug
EUR 363
Description: Mouse monoclonal to PRDM1/Blimp1

PRDM1 Recombinant Protein (Human)

RP042457 100 ug Ask for price

PRDM1 Recombinant Protein (Mouse)

RP164228 100 ug Ask for price

PRDM1 Recombinant Protein (Rat)

RP221939 100 ug Ask for price

Anti-PRDM1/MEL1 Antibody

A00412 100ug/200ul
EUR 397
Description: Goat Polyclonal PRDM1/MEL1 Antibody. Validated in IHC, WB and tested in Human, Mouse, Rat.

Anti-PRDM1/Blimp1 Antibody

A00412-1 100ug/vial
EUR 334

Anti-PRDM1 BLIMP1 Antibody

A00412-2 100ug
EUR 455
Description: Rabbit Polyclonal PRDM1 BLIMP1 Antibody. Validated in IP, IHC, WB and tested in Human, Mouse, Rat.

Human PRDM1 shRNA Plasmid

20-abx950435
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg

Mouse PRDM1 shRNA Plasmid

20-abx969359
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg

Anti-PRDM1 / MEL1 antibody

STJ72074 100 µg
EUR 359

Anti-PRDM1 / BLIMP1 antibody

STJ70276 100 µg
EUR 359

Prdm1 ORF Vector (Rat) (pORF)

ORF073981 1.0 ug DNA
EUR 506

PRDM1 ORF Vector (Human) (pORF)

ORF014153 1.0 ug DNA
EUR 354

Prdm1 ORF Vector (Mouse) (pORF)

ORF054744 1.0 ug DNA
EUR 506

pECMV-Prdm1-m-FLAG Plasmid

PVT15439 2 ug
EUR 325

Monoclonal PRDM1 Antibody, Clone: 5E7

AMM02750G 0.1ml
EUR 484
Description: A Monoclonal antibody against Human PRDM1. The antibodies are raised in Mouse and are from clone 5E7. This antibody is applicable in WB, E

Monoclonal PRDM1 Antibody, Clone: 7C11H1

AMM03066G 0.1ml
EUR 528
Description: A Monoclonal antibody against Human PRDM1. The antibodies are raised in Mouse and are from clone 7C11H1. This antibody is applicable in WB and IHC, FC, ICC, E

Monoclonal PRDM1 Antibody, Clone: 2F1B6

AMM03067G 0.1ml
EUR 528
Description: A Monoclonal antibody against Human PRDM1. The antibodies are raised in Mouse and are from clone 2F1B6. This antibody is applicable in WB and IHC, FC, ICC, E

Polyclonal PRDM1 Antibody (N-term)

APR03968G 0.1ml
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human PRDM1 (N-term). This antibody is tested and proven to work in the following applications:

Polyclonal BLIMP1 / PRDM1 Antibody (Internal)

APG01185G 0.05mg
EUR 484
Description: A polyclonal antibody raised in Goat that recognizes and binds to Human BLIMP1 / PRDM1 (Internal). This antibody is tested and proven to work in the following applications:

Prdm1 sgRNA CRISPR Lentivector set (Rat)

K6508201 3 x 1.0 ug
EUR 339

Prdm1 sgRNA CRISPR Lentivector set (Mouse)

K3985501 3 x 1.0 ug
EUR 339

PRDM1 sgRNA CRISPR Lentivector set (Human)

K1715601 3 x 1.0 ug
EUR 339

PR/SET Domain 1 (PRDM1) Antibody

abx037111-100ug 100 ug
EUR 391

PR/SET Domain 1 (PRDM1) Antibody

abx011375-100ul 100 ul
EUR 411

PR/SET Domain 1 (PRDM1) Antibody

abx016176-100ug 100 ug
EUR 411

PR/SET Domain 1 (PRDM1) Antibody

abx028112-400ul 400 ul
EUR 523

PR/SET Domain 1 (PRDM1) Antibody

abx028112-80l 80 µl
EUR 286

PR/SET Domain 1 (PRDM1) Antibody

abx430328-200ul 200 ul
EUR 384

PR/SET Domain 1 (PRDM1) Antibody

abx430329-200ul 200 ul
EUR 384

Polyclonal BLIMP1 / PRDM1 Antibody (C-Terminus)

APR02526G 0.05mg
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human BLIMP1 / PRDM1 (C-Terminus). This antibody is tested and proven to work in the following applications:

Polyclonal PRDM1 / BLIMP1 Antibody (C-Term)

APG00388G 0.1mg
EUR 484
Description: A polyclonal antibody raised in Goat that recognizes and binds to Human PRDM1 / BLIMP1 (C-Term). This antibody is tested and proven to work in the following applications:

Polyclonal PRDM1 / MEL1 Antibody (internal region)

APG00624G 0.1mg
EUR 484
Description: A polyclonal antibody raised in Goat that recognizes and binds to Human PRDM1 / MEL1 (internal region). This antibody is tested and proven to work in the following applications:

PR/SET Domain 1 (PRDM1) Antibody

abx332134-100ul 100 ul
EUR 425

PRDM1 3'UTR Luciferase Stable Cell Line

TU018764 1.0 ml
EUR 2333

Prdm1 3'UTR Luciferase Stable Cell Line

TU116893 1.0 ml Ask for price

Prdm1 3'UTR GFP Stable Cell Line

TU166893 1.0 ml Ask for price

Prdm1 3'UTR Luciferase Stable Cell Line

TU216727 1.0 ml Ask for price

Prdm1 3'UTR GFP Stable Cell Line

TU266727 1.0 ml Ask for price

PRDM1 3'UTR GFP Stable Cell Line

TU068764 1.0 ml
EUR 2333

PRDM1 Protein Vector (Rat) (pPB-C-His)

PV295922 500 ng
EUR 1191

PRDM1 Protein Vector (Rat) (pPB-N-His)

PV295923 500 ng
EUR 1191

PRDM1 Protein Vector (Rat) (pPM-C-HA)

PV295924 500 ng
EUR 1191

 

 

Monoclonal PRDM1 Antibody (monoclonal) (M01), Clone: 2B10

Description: A Monoclonal antibody towards Human PRDM1 (monoclonal) (M01). The antibodies are raised in mouse and are from clone 2B10. This antibody is relevant in WB, E

Aptamer-directed Protein-specific A number of Modifications of Membrane Glycoproteins on Dwelling Cells

Understanding how a cell membrane protein capabilities on dwelling cells stays a problem for cell biology. Particular placement of practical molecules on particular proteins of their native atmosphere would enable complete research of proteins’ dynamic capabilities.

Present strategies can’t facilely obtain a number of modifications on particular membrane proteins. On this report, we describe an aptamer-induced, protein-specific bioorthogonal modification expertise for exact non-genetic immobilization of a number of small practical molecules heading in the right direction membrane glycoproteins by combining metabolic expertise and aptamer focusing on.

In briefly, DNA probes had been designed by modifying aptamers, which bind to focus on proteins on the surfaces of dwelling cells pretreated with N-azidoacetylmannosamine-tetraacylated (Ac4ManNAz). The cyclooctynes tagged of DNA probes will strategy the azide teams to set off the bioorthogonal reactions.

After UV irradiation and hybridization with cDNA (complementary DNA), the aptamers will be eliminated, and the method will be repeated to attain a number of modifications for multicolor imaging and cell floor nano-engineering on particular proteins.

 

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