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Top VHL Recombinant Protein For Human
Von Hippel-Lindau (VHL) Protein Antagonist VH298 Improves Wound Therapeutic in Streptozotocin-Induced Hyperglycaemic Rats by Activating Hypoxia-Inducible Issue- (HIF-) 1 Signalling.
The aim of the current analysis is to analyze the results of the VHL protein antagonist, VH298, on purposeful actions of fibroblasts and vascular endothelial cells and the results on the wound therapeutic course of in a streptozotocin-induced hyperglycaemic rat mannequin.
Strategies
HIF-1α and hydroxy-HIF-1α protein ranges in VH298-treated rat fibroblasts (rFb) had been measured by immunoblotting, rFb proliferation was detected by the CCK-Eight assay, and mRNA ranges of associated genes had been measured by quantitative RT-PCR. In vitro wound therapeutic was simulated by the scratch take a look at; angiogenesis was measured by the human umbilical vein endothelial cell (hUVEC) tube formation assay. VH298 or PBS was regionally injected into wounds in rat fashions with streptozotocbd tissues had been harvested, and haematoxylin-eosin (HE) and Masson trichrome staining and immunohistochemical processes had been carried out.
Human Von Hippel Lindau Tumor Suppressor (vHL) ELISA Equipment
Outcomes
HIF-1α and hydroxy-HIF-1α ranges elevated in VH298-treated rFb, in a time- and dose-dependent method. Thirty micromolar VH298 may considerably enhance cell proliferation, angiogenesis, and gene expression of sort I collagen-αα
Conclusions
Taken collectively, VH298 activated the HIF-1 signalling pathway by stabilizing each HIF-1α and hydroxy-HIF-1α. VH298 enhanced rFb capabilities, promoted hUVEC angiogenesis, and accelerated wound therapeutic within the rat mannequin mimicking diabetes mellitus.
Rat Von Hippel Lindau Tumor Suppressor (vHL) ELISA Equipment

VHL Antibody |
1-CSB-PA991849 |
Cusabio |
|
|
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Description: A polyclonal antibody against VHL. Recognizes VHL from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:2000-1:10000, IHC:1:50-1:200 |
VHL Antibody |
1-CSB-PA060067 |
Cusabio |
|
|
|
Description: A polyclonal antibody against VHL. Recognizes VHL from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: IHC, ELISA;IHC:1/100-1/300.ELISA:1/40000 |
VHL Antibody |
1-CSB-PA071125 |
Cusabio |
|
|
|
Description: A polyclonal antibody against VHL. Recognizes VHL from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:2000-1:5000, WB:1:500-1:2000, IHC:1:25-1:100 |
VHL Antibody |
1-CSB-PA876839 |
Cusabio |
|
|
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Description: A polyclonal antibody against VHL. Recognizes VHL from Human. This antibody is Unconjugated. Tested in the following application: IHC, ELISA;IHC-p:1:50-300, ELISA:1:10000-20000 |
VHL Antibody |
CSB-PA025852KA01HU- |
Cusabio |
each |
EUR 402 |
|
Description: A polyclonal antibody against VHL. Recognizes VHL from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:2000, IHC:1:50-1:200 |
VHL Antibody |
CSB-PA025852KA01HU-100ul |
Cusabio |
100ul |
EUR 466.8 |
|
Description: A polyclonal antibody against VHL. Recognizes VHL from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:2000, IHC:1:50-1:200 |
VHL Antibody |
F49681-0.05ML |
NSJ Bioreagents |
0.05 ml |
EUR 140.25 |
Description: Von Hippel-Lindau syndrome (VHL) is a dominantly inherited familial cancer syndrome predisposing to a variety of malignant and benign tumors. A germline mutation of VHL gene is the basis of familial inheritance of VHL syndrome. The protein is a component of the protein complex that includes elongin B, elongin C, and cullin-2, and possesses ubiquitin ligase E3 activity. This protein is involved in the ubiquitination and degradation of hypoxia-inducible-factor (HIF), which is a transcription factor that plays a central role in the regulation of gene expression by oxygen. RNA polymerase II subunit POLR2G/RPB7 is also reported to be a target of this protein. |
VHL Antibody |
F49681-0.2ML |
NSJ Bioreagents |
0.2 ml |
EUR 322.15 |
Description: Von Hippel-Lindau syndrome (VHL) is a dominantly inherited familial cancer syndrome predisposing to a variety of malignant and benign tumors. A germline mutation of VHL gene is the basis of familial inheritance of VHL syndrome. The protein is a component of the protein complex that includes elongin B, elongin C, and cullin-2, and possesses ubiquitin ligase E3 activity. This protein is involved in the ubiquitination and degradation of hypoxia-inducible-factor (HIF), which is a transcription factor that plays a central role in the regulation of gene expression by oxygen. RNA polymerase II subunit POLR2G/RPB7 is also reported to be a target of this protein. |
VHL Antibody |
F49682-0.08ML |
NSJ Bioreagents |
0.08 ml |
EUR 140.25 |
Description: Von Hippel-Lindau syndrome (VHL) is a dominantly inherited familial cancer syndrome predisposing to a variety of malignant and benign tumors. A germline mutation of VHL gene is the basis of familial inheritance of VHL syndrome. The protein is a component of the protein complex that includes elongin B, elongin C, and cullin-2, and possesses ubiquitin ligase E3 activity. This protein is involved in the ubiquitination and degradation of hypoxia-inducible-factor (HIF), which is a transcription factor that plays a central role in the regulation of gene expression by oxygen. RNA polymerase II subunit POLR2G/RPB7 is also reported to be a target of this protein. |
VHL Antibody |
F49682-0.4ML |
NSJ Bioreagents |
0.4 ml |
EUR 330.65 |
Description: Von Hippel-Lindau syndrome (VHL) is a dominantly inherited familial cancer syndrome predisposing to a variety of malignant and benign tumors. A germline mutation of VHL gene is the basis of familial inheritance of VHL syndrome. The protein is a component of the protein complex that includes elongin B, elongin C, and cullin-2, and possesses ubiquitin ligase E3 activity. This protein is involved in the ubiquitination and degradation of hypoxia-inducible-factor (HIF), which is a transcription factor that plays a central role in the regulation of gene expression by oxygen. RNA polymerase II subunit POLR2G/RPB7 is also reported to be a target of this protein. |
VHL Antibody |
R35773-100UG |
NSJ Bioreagents |
100 ug |
EUR 339.15 |
Description: Additional name(s) for this target protein: Von Hippel-Lindau tumor suppressor, VHL1 |
VHL Antibody |
E90377 |
EnoGene |
100ul |
EUR 255 |
Description: Available in various conjugation types. |
VHL Antibody |
E19-6104 |
EnoGene |
100μg/100μl |
EUR 225 |
Description: Available in various conjugation types. |
VHL Antibody |
E18-6292-1 |
EnoGene |
50μg/50μl |
EUR 145 |
Description: Available in various conjugation types. |
VHL Antibody |
E18-6292-2 |
EnoGene |
100μg/100μl |
EUR 225 |
Description: Available in various conjugation types. |
VHL Antibody |
E312859 |
EnoGene |
200ul |
EUR 275 |
Description: Available in various conjugation types. |
anti- VHL antibody |
FNab09402 |
FN Test |
100µg |
EUR 658.5 |
|
Description: Antibody raised against VHL |
anti- VHL antibody |
FNab10175 |
FN Test |
100µg |
EUR 606.3 |
|
Description: Antibody raised against VHL |
Human VHL Antibody |
32825-05111 |
AssayPro |
150 ug |
EUR 313.2 |
VHL (pS68) Antibody |
abx219320-100ug |
Abbexa |
100 ug |
EUR 526.8 |
|
VHL Conjugated Antibody |
C32075 |
SAB |
100ul |
EUR 476.4 |
VHL Polyclonal Antibody |
46866-100ul |
SAB |
100ul |
EUR 302.4 |
VHL Polyclonal Antibody |
46866-50ul |
SAB |
50ul |
EUR 224.4 |
VHL Polyclonal Antibody |
ABP56500-003ml |
Abbkine |
0.03ml |
EUR 189.6 |
|
Description: A polyclonal antibody for detection of VHL from Human, Mouse, Rat. This VHL antibody is for IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human VHL around the non-phosphorylation site of S68 |
VHL Polyclonal Antibody |
ABP56500-01ml |
Abbkine |
0.1ml |
EUR 346.8 |
|
Description: A polyclonal antibody for detection of VHL from Human, Mouse, Rat. This VHL antibody is for IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human VHL around the non-phosphorylation site of S68 |
VHL Polyclonal Antibody |
ABP56500-02ml |
Abbkine |
0.2ml |
EUR 496.8 |
|
Description: A polyclonal antibody for detection of VHL from Human, Mouse, Rat. This VHL antibody is for IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human VHL around the non-phosphorylation site of S68 |
VHL Polyclonal Antibody |
ABP60891-003ml |
Abbkine |
0.03ml |
EUR 189.6 |
|
Description: A polyclonal antibody for detection of VHL from Human. This VHL antibody is for IHC-P, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human VHL protein at amino acid sequence of 1-50 |
VHL Polyclonal Antibody |
ABP60891-01ml |
Abbkine |
0.1ml |
EUR 346.8 |
|
Description: A polyclonal antibody for detection of VHL from Human. This VHL antibody is for IHC-P, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human VHL protein at amino acid sequence of 1-50 |
VHL Polyclonal Antibody |
ABP60891-02ml |
Abbkine |
0.2ml |
EUR 496.8 |
|
Description: A polyclonal antibody for detection of VHL from Human. This VHL antibody is for IHC-P, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human VHL protein at amino acid sequence of 1-50 |
VHL Polyclonal Antibody |
ES7499-100ul |
ELK Biotech |
100ul |
EUR 334.8 |
Description: A Rabbit Polyclonal antibody against VHL from Human/Mouse/Rat. This antibody is tested and validated for IHC, WB, ELISA |
VHL Polyclonal Antibody |
ES7499-50ul |
ELK Biotech |
50ul |
EUR 248.4 |
Description: A Rabbit Polyclonal antibody against VHL from Human/Mouse/Rat. This antibody is tested and validated for IHC, WB, ELISA |
VHL Polyclonal Antibody |
ES8746-100ul |
ELK Biotech |
100ul |
EUR 334.8 |
Description: A Rabbit Polyclonal antibody against VHL from Human. This antibody is tested and validated for IHC, WB, ELISA |
VHL Polyclonal Antibody |
ES8746-50ul |
ELK Biotech |
50ul |
EUR 248.4 |
Description: A Rabbit Polyclonal antibody against VHL from Human. This antibody is tested and validated for IHC, WB, ELISA |
VHL Polyclonal Antibody |
E916287 |
EnoGene |
100ul |
EUR 225 |
Description: Available in various conjugation types. |
VHL Polyclonal Antibody |
E911240 |
EnoGene |
100ul |
EUR 225 |
Description: Available in various conjugation types. |
VHL Polyclonal Antibody |
E046866 |
EnoGene |
100μg/100μl |
EUR 255 |
Description: Available in various conjugation types. |
VHL Polyclonal Antibody |
E20-74876 |
EnoGene |
100ug |
EUR 225 |
Description: Available in various conjugation types. |
VHL Polyclonal Antibody |
E11-2035988 |
EnoGene |
100ug/100ul |
EUR 225 |
Description: Available in various conjugation types. |
VHL (Ab-68) Antibody |
E11-8450B |
EnoGene |
100μg |
EUR 225 |
Description: Available in various conjugation types. |
Polyclonal VHL Antibody (C-term) |
APR10723G |
Leading Biology |
0.1ml |
EUR 580.8 |
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human VHL (C-term). This antibody is tested and proven to work in the following applications: |
VHL Dependent Expression of REDD1 and PDK3 Proteins in Clear-cell Renal Cell Carcinoma.
- Sporadic clear-cell renal cell carcinoma (ccRCC) is related with mutations within the VHL gene, upregulated mammalian goal of rapamycin (mTOR) exercise and glycolytic metabolism. Right here, we analyze the impact of VHL mutational standing on the expression stage of mTOR, eIF4E-BP1, AMPK, REDD1, and PDK3 proteins.
pGF-AP1-mCMV-EF1-Puro (293 secure cell line)
- Complete proteins had been remoted from 21 tumorous samples with biallelic inactivation, 10 with monoallelic inactivation and 6 tumors with a wild-type VHL (wtVHL) gene obtained from sufferers who underwent complete nephrectomy. The expressions of goal proteins had been assessed utilizing Western blot.Expressions of mTOR, eIF4EBP1 and AMPOkay had been VHL impartial.
- Tumors with monoallelic inactivation of VHL underexpressed REDD1 compared to wtVHL tumors (P = 0.042), tumors with biallelic VHL inactivation (P < 0.005) and management tissue (P = 0.004). Moreover, REDD1 expression was larger in tumors with VHL biallelic inactivation than in management tissue (P = 0.008). Solely in wt tumor samples PDK3 was overexpressed compared to tumors with biallelic inactivation of VHL gene (P = 0.012) and controls (P = 0.016). In wtVHL ccRCC, multivariate linear regression evaluation revealed that 97.4% of variability in PDK3 expression might be defined by variations in AMPK quantity.Expressions of mTOR, eIF4EBP1 and AMPK had been VHL impartial.
- Now we have proven for the primary time VHL dependent expression of PDK3 and we offer extra proof that VHL mutational standing impacts REDD1 expression in sporadic ccRCC.
Gene Knock-Out HR Concentrating on Vector w/Single Choice Marker (Blasticidin) and Destructive Choice (TK) Towards Random Integration
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VHL sufferers (n = 339) had been recruited and grouped primarily based on mutation sorts: HIF-α binding web site missense (HM) mutations, non-HIF-α binding web site missense (nHM) mutations, and truncating (TR) mutations. Age-related dangers of VHL-associated tumors and affected person survival had been in contrast.
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Missense mutations conferred an elevated danger of pheochromocytoma (HR = 1.854, p = 0.047) in contrast with truncating mutations. The danger of pheochromocytoma was decrease within the HM group than within the nHM group (HR = 0.298, p = 0.003) however was related between HM and TR teams (HR = 0.901, p = 0.810).
TARGATT? Knock-in HEK293 Generation Kit (Master Cell Line) |
AST-1300 |
Applied StemCell |
1 Kit |
Ask for price |
Description: 12 month |
B2M Knockout Jurkat Cell Line |
78342 |
BPS Bioscience |
2 vials |
EUR 6500 |
Description: B2M (Beta-2-Microglobulin) has been genetically removed by CRISPR/Cas9 genome editing from Jurkat cells. |
TCR Knockout Jurkat Cell Line |
78539 |
BPS Bioscience |
2 vials |
EUR 6500 |
Description: The TCR Knockout Jurkat cell line was generated by CRISPR/Cas9 genome editing to remove the TRAC (T-Cell Receptor Alpha Constant) and TRBC1 (T-Cell Receptor Beta Constant 1) domains of the TCRα and β chains. |
293AD Cell Line |
AD-100 |
Cell Biolabs |
1 vial |
EUR 553.2 |
Description: The 293AD Cell Line is derived from the parental 293 cells but selected for attributes that increase adenovirus production, including firmer attachment and larger surface area. |
293AAV Cell Line |
AAV-100 |
Cell Biolabs |
1 vial |
EUR 609.6 |
Description: The 293AAV Cell Line is derived from the parental 293 cells but selected for attributes that increase AAV production, including firmer attachment and larger surface area. |
293LTV Cell Line |
LTV-100 |
Cell Biolabs |
1 vial |
EUR 609.6 |
Description: The 293LTV Cell Line is derived from the parental 293 cells but selected for attributes that increase lentiviral production, including fimrer attachment and larger surface area. |
293RTV Cell Line |
RV-100 |
Cell Biolabs |
1 vial |
EUR 609.6 |
Description: The 293RTV Cell Line is derived from the parental 293 cells but selected for attributes that increase retroviral production, including fimrer attachment and larger surface area. |
B2M Knockout THP-1 Cell Line |
78389 |
BPS Bioscience |
2 vials |
EUR 6500 |
Description: B2M (Beta-2-Microglobulin) has been genetically removed by CRISPR/Cas9 genome editing from THP-1 cells. |
CIITA Knockout THP-1 Cell Line |
78390 |
BPS Bioscience |
2 vials |
EUR 6500 |
Description: CIITA (Class II Transactivator) has been genetically removed from THP-1 cells using CRISPR/Cas9 genome editing. |
TCR/B2M Knockout Jurkat Cell Line |
78552 |
BPS Bioscience |
2 vials |
EUR 8645 |
Description: This cell line is a double knockout of TCR (T Cell Receptor) and B2M (Beta-2-Microglobulin). First, the TRAC (T-Cell Receptor Alpha Constant) and the TRBC1 (T-Cell Receptor Beta Constant 1) domains of the TCRα/β chains were genetically removed by CRISPR/Cas9 genome editing from Jurkat cells to generate the TCR Knockout Jurkat cell Line (BPS Bioscience #78539). These TCR knockout cells were then used to generate a new cell line in which B2M was also genetically removed by CRISPR/Cas9 genome editing. |
T47D/GFP Cell Line |
AKR-208 |
Cell Biolabs |
1 vial |
EUR 686.4 |
Description: T47D/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line. |
A549/GFP Cell Line |
AKR-209 |
Cell Biolabs |
1 vial |
EUR 686.4 |
Description: A549/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line. |
HeLa/GFP Cell Line |
AKR-213 |
Cell Biolabs |
1 vial |
EUR 686.4 |
Description: HeLa/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line. |
NIH3T3/GFP Cell Line |
AKR-214 |
Cell Biolabs |
1 vial |
EUR 686.4 |
Description: NIH3T3/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line. |
NIH3T3/Cas9 Cell Line |
AKR-5104 |
Cell Biolabs |
1 vial |
EUR 686.4 |
MCF-7/Luc Cell Line |
AKR-234 |
Cell Biolabs |
1 vial |
EUR 686.4 |
Description: MCF-7/Luc Cell Line stably expresses luciferase and otherwise exhibits the same characteristics of the parental cell line. |
SKOV-3/Luc Cell Line |
AKR-232 |
Cell Biolabs |
1 vial |
EUR 686.4 |
Description: SKOV-3/Luc Cell Line stably expresses luciferase and otherwise exhibits the same characteristics of the parental cell line. |
FCGR2A (CD32A) Knockout Jurkat Cell Line |
78549 |
BPS Bioscience |
2 vials |
EUR 6500 |
Description: The FCGR2A Knockout Jurkat cell line was generated by CRISPR/Cas9 genome editing to remove FCGR2A (CD32A), the gene encoding protein FcγRIIa (Fragment crystallizable gamma receptor II a, also known as FcGRIIa, Fc-gamma-RIIa, and CD32A). |
OVCAR-5/RFP Cell Line |
AKR-254 |
Cell Biolabs |
1 vial |
EUR 686.4 |
Description: OVCAR-5/RFP Cell Line stably expresses RFP and otherwise exhibits the same characteristics of the parental cell line. |
B2M/CIITA Double Knockout THP-1 Cell Line |
78391 |
BPS Bioscience |
2 vials |
EUR 9500 |
Description: Both B2M (Beta-2-Microglobulin) and CIITA (Class II Transactivator) have been genetically removed from THP-1 cells using CRISPR/Cas9 genome editing. |
Gene Knock-Out HR Targeting Vector [MCS1-EF1α-RFP-T2A-Puro-pA-MCS2] |
HR110PA-1 |
SBI |
10 ug |
EUR 933 |
Gene Knock-Out HR Targeting Vector [MCS1-EF1a-GFP-T2A-Puro-pA-MCS2] |
HR410PA-1 |
SBI |
10 ug |
EUR 933 |
Gene Knock-Out HR Targeting Vector [MCS1-EF1a-RFP-T2A-Hygro-pA-MCS2] |
HR510PA-1 |
SBI |
10 ug |
EUR 933 |
Platinum-E Retroviral Packaging Cell Line, Ecotropic |
RV-101 |
Cell Biolabs |
1 vial |
EUR 1104 |
Description: Conventional cells used for retrovirus packaging, such as those based on NIH3T3 cells, have limited stability and produce relatively low yields of retrovirus, mainly due to the poor expression of retroviral structure proteins (gag, pol and env) in the cells. The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line. They exhibit longer stability and produce higher yields of retroviral structure proteins. Plat-E cells contain gag, pol and env genes, allowing retroviral packaging with a single plasmid transfection. |
Total Protein - Murine Embryonic Stem Cell Line D3 |
CBA-305 |
Cell Biolabs |
500 ?g |
EUR 414 |
Description: - Isolated from mouse ES-D3 cell line
- Presented as 500 µg at 1 mg/mL in NP-40 Solubilization Buffer
|
B2M Knockout NFAT Luciferase Reporter Jurkat Cell Line |
78363 |
BPS Bioscience |
2 vials |
EUR 11095 |
Description: B2M (Beta-2-Microglobulin) has been genetically removed by CRISPR/Cas9 genome editing from NFAT Luciferase Reporter Jurkat cells. Expression of the firefly luciferase gene is driven by NFAT response elements located upstream of the minimal TATA promoter. Activation of the NFAT signaling pathway in these cells can be monitored by measuring luciferase activity. |
Platinum-GP Retroviral Packaging Cell Line, Pantropic |
RV-103 |
Cell Biolabs |
1 vial |
EUR 1104 |
Description: Conventional cells used for retrovirus packaging, such as those based on NIH3T3 cells, have limited stability and produce relatively low yields of retrovirus, mainly due to the poor expression of retroviral structure proteins (gag, pol and env) in the cells. The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line. They exhibit longer stability and produce higher yields of retroviral structure proteins. Plat-GP cells contain the gag and pol genes required for retroviral packaging; an expression vector is co-transfected with a VSVG envelope vector. |
Platinum-A Retroviral Packaging Cell Line, Amphotropic |
RV-102 |
Cell Biolabs |
1 vial |
EUR 1104 |
Description: Conventional cells used for retrovirus packaging, such as those based on NIH3T3 cells, have limited stability and produce relatively low yields of retrovirus, mainly due to the poor expression of retroviral structure proteins (gag, pol and env) in the cells. The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line. They exhibit longer stability and produce higher yields of retroviral structure proteins. Plat-A cells contain gag, pol and env genes, allowing retroviral packaging with a single plasmid transfection. |
TCR Knockout NFAT-Luciferase Reporter Jurkat Cell Line |
78556 |
BPS Bioscience |
2 vials |
EUR 12205 |
Description: This cell line is a knockout of TCR (T Cell Receptor). The TRAC (T-Cell Receptor Alpha Constant) and TRBC1 (T-Cell Receptor Beta Constant 1) domains of the TCRα/β chains were genetically removed by CRISPR/Cas9 genome editing from recombinant Jurkat cells stably expressing the firefly luciferase gene under the control of NFAT response elements.This cell line has been functionally validated and does not respond to anti-CD3 agonist antibodies, as opposed to parental NFAT-Luciferase Reporter Jurkat cells (BPS Bioscience #60621). |
TCR/B2M Knockout NFAT Luciferase Reporter Jurkat Cell Line |
78557 |
BPS Bioscience |
2 vials |
EUR 16695 |
Description: This cell line is a double knockout of TCR (T Cell Receptor) and B2M (Beta-2-Microglobulin). First, the TRAC (T-Cell Receptor Alpha Constant) and the TRBC1 (T-Cell Receptor Beta Constant 1) domains of the TCRα/β chains were genetically removed by CRISPR/Cas9 genome editing from NFAT Luciferase Reporter Jurkat cells to generate the TCR Knockout NFAT Luciferase Reporter Jurkat cell Line (BPS Bioscience #78556). These TCR knockout cells were used to generate a new cell line in which B2M was also genetically removed by CRISPR/Cas9 genome editing. _x000D_Expression of the firefly luciferase gene is driven by NFAT response elements located upstream of the minimal TATA promoter. Activation of the NFAT signaling pathway in these cells can be monitored by measuring luciferase activity. |
TARGATT? Knock-in iPSC Quick Knockin Kit |
AST-1101 |
Applied StemCell |
1 Kit |
Ask for price |
Description: 12 month |
Basic HR Targeting Vector [MCS1-LoxP-MCS2-MCS3-pA-LoxP-MCS4] for Gene Knock-In/Out |
HR100PA-1 |
SBI |
10 ug |
EUR 868 |
TARGATT? Knock-in iPSC Genotyping Kit |
AST-1102 |
Applied StemCell |
1 Kit |
Ask for price |
Description: 12 month |
293 [HEK-293] Cell Line |
CL-0001 |
Elabscience Biotech |
1×10⁶ cells/vial |
EUR 420 |
|
Description: Homo sapiens, Human |
Gene Knock-Out HR Targeting Vector w/Single Selection Marker (Blasticidin) and Negative Selection (TK) Against Random Integration |
HR720PA-1 |
SBI |
10 µg |
EUR 1071 |
Vhl 3'UTR GFP Stable Cell Line |
TU273038 |
ABM |
1.0 ml |
Ask for price |
VHL 3'UTR GFP Stable Cell Line |
TU078117 |
ABM |
1.0 ml |
EUR 1825.2 |
Vhl 3'UTR GFP Stable Cell Line |
TU171762 |
ABM |
1.0 ml |
Ask for price |
Gene Knock-Out HR Targeting Vector w/Dual Selection Markers (GFP+Puro) and Negative Selection (TK) Against Random Integration |
HR700PA-1 |
SBI |
10 µg |
EUR 1071 |
Gene Knock-Out HR Targeting Vector w/Dual Selection Markers (RFP+Hygro) and Negative Selection (TK) Against Random Integration |
HR710PA-1 |
SBI |
10 µg |
EUR 1071 |
Gene Knock-Out HR Targeting Vector with TK selection [MCS1-LoxP-EF1α-GFP-T2A-Puro-P2A-hsvTK-pA-LoxP-MCS2] |
HR210PA-1 |
SBI |
10 ug |
EUR 1071 |
Vhl 3'UTR Luciferase Stable Cell Line |
TU121762 |
ABM |
1.0 ml |
Ask for price |
VHL 3'UTR Luciferase Stable Cell Line |
TU028117 |
ABM |
1.0 ml |
EUR 1825.2 |
Vhl 3'UTR Luciferase Stable Cell Line |
TU223038 |
ABM |
1.0 ml |
Ask for price |
StemTAG Stem Cell Colony Formation Assay (Cell Recovery Compatible) |
CBA-325 |
Cell Biolabs |
96 assays |
EUR 1027.2 |
Description: Our StemTAG 96-Well Stem Cell Colony Formation Assay provides a high-throughput method to quantify ES cells in just 7-10 days, and no manual cell counting is required. Once colonies are formed, they may be analyzed in three different ways: 1. Lyse cells, then quantify in a fluorescence plate reader using dye included in the kit; 2. Lyse cells, then quantify alkaline phosphatase activity using reagents provided; or 3. Recover colonies from matrix for further culture or analysis. |
StemTAG Stem Cell Colony Formation Assay (Cell Recovery Compatible) |
CBA-325-5 |
Cell Biolabs |
5 x 96 assays |
EUR 4033.2 |
Description: Our StemTAG 96-Well Stem Cell Colony Formation Assay provides a high-throughput method to quantify ES cells in just 7-10 days, and no manual cell counting is required. Once colonies are formed, they may be analyzed in three different ways: 1. Lyse cells, then quantify in a fluorescence plate reader using dye included in the kit; 2. Lyse cells, then quantify alkaline phosphatase activity using reagents provided; or 3. Recover colonies from matrix for further culture or analysis. |
CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Colorimetric |
CBA-135 |
Cell Biolabs |
96 assays |
EUR 985.2 |
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis. |
CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Colorimetric |
CBA-135-5 |
Cell Biolabs |
5 x 96 assays |
EUR 4027.2 |
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis. |
CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Fluorometric |
CBA-140 |
Cell Biolabs |
96 assays |
EUR 1027.2 |
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis. |
CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Fluorometric |
CBA-140-5 |
Cell Biolabs |
5 x 96 assays |
EUR 4179.6 |
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis. |
AAV-293 Cell Line |
CL-0019 |
Elabscience Biotech |
1×10⁶ cells/vial |
EUR 420 |
|
Description: Homo sapiens, Human |
CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Colorimetric, Trial Size |
CBA-135-T |
Cell Biolabs |
24 assays |
EUR 518.4 |
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis. |
CytoSelect 96-Well Cell Transformation Assay (Cell Recovery Compatible, Fluorometric), Trial Size |
CBA-140-T |
Cell Biolabs |
24 assays |
EUR 547.2 |
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis. |
Collagen-based Cell Contraction Assay |
CBA-201 |
Cell Biolabs |
24 assays |
EUR 582 |
Description: Cell Biolabs? Collagen-based Contraction Assay Kit provides a simple system to assess cell contractivity in vitro and screen cell contraction mediators. Each kit provides sufficient quantities to perform up to 24 assays in a 24-well plate. The kit can be also used in culturing cells in 3D collagen matrix. |
CytoSelect MTT Cell Proliferation Assay |
CBA-252 |
Cell Biolabs |
960 assays |
EUR 490.8 |
Description: Cell Biolabs? CytoSelect MTT Cell Proliferation Assay provides a colorimetric format for measuring and monitoring cell proliferation. The kit contains sufficient reagents for the evaluation of 960 assays in 96-well plates or 192 assays in 24-well plates. Cells can be plated and then treated with compounds or agents that affect proliferation. Cells are then detected with the proliferation reagent, which is converted in live cells from the yellow tetrazole MTT to the purple formazan form by a cellular reductase (Figure 1). An increase in cell proliferation is accompanied by an increased signal, while a decrease in cell proliferation (and signal) can indicate the toxic effects of compounds or suboptimal culture conditions. The assay principles are basic and can be applied to most eukaryotic cell lines, including adherent and non-adherent cells and certain tissues. This cell proliferation reagent can be used to detect proliferation in bacteria, yeast, fungi, protozoa as well as cultured mammalian and piscine cells. |
HIF-1 Alpha Cell Based ELISA Kit |
CBA-281 |
Cell Biolabs |
96 assays |
EUR 734.4 |
Description: Cell Biolabs? HIF-1 Cell Based ELISA Kit is an immunoassay developed for rapid detection of HIF-1 Alpha in fixed cells. Cells on a microplate are stimulated for HIF-1 Alpha stabilization, fixed, permeabilized, and then neutralized in the well. HIF-1 Alpha is then detected with an anti-HIF-1 alpha antibody followed by an HRP conjugated secondary antibody. Each kit provides sufficient reagents to perform up to a total of 96 assays and can detect HIF-1 Alpha from human, mouse, or rat. |
Radius 24-Well Cell Migration Assay |
CBA-125 |
Cell Biolabs |
24 assays |
EUR 602.4 |
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap. |
Radius 24-Well Cell Migration Assay |
CBA-125-5 |
Cell Biolabs |
5 x 24 assays |
EUR 2362.8 |
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap. |
Radius 96-Well Cell Migration Assay |
CBA-126 |
Cell Biolabs |
96 assays |
EUR 686.4 |
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap. |
Radius 96-Well Cell Migration Assay |
CBA-126-5 |
Cell Biolabs |
5 x 96 assays |
EUR 2697.6 |
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap. |
Radius 384-Well Cell Migration Assay |
CBA-127 |
Cell Biolabs |
384 assays |
EUR 721.2 |
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap. |
Radius 384-Well Cell Migration Assay |
CBA-127-5 |
Cell Biolabs |
5 x 384 wells |
EUR 2802 |
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap. |
Radius 48-Well Cell Migration Assay |
CBA-5037 |
Cell Biolabs |
48 assays |
EUR 622.8 |
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap. |
Radius 48-Well Cell Migration Assay |
CBA-5037-5 |
Cell Biolabs |
5 x 48 assays |
EUR 2454 |
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap. |
CFTR - HEK 293 Cell Line |
60506 |
BPS Bioscience |
2 vials |
EUR 7500 |
Description: The CFTR HEK293 Cell Line expresses full length, wild-type human cystic fibrosis transmembrane conductance regulator (CFTR) protein (Genbank #P13569, NP_000483.3), with a C-terminal Streptavidin-Binding Peptide (SBP) tag.Expression must be induced ≥24 hours prior to an experiment using 1 µg/ml Doxycycline and 3 mM Na-butyrate. The inducible expression of CFTR was confirmed by Western blotting and flow cytometry. |
CytoSelect BrdU Cell Proliferation ELISA Kit |
CBA-251 |
Cell Biolabs |
96 assays |
EUR 637.2 |
Description: The CytoSelect BrdU Cell Proliferation ELISA Kit detects BrdU incorporated into cellular DNA during cell proliferation using an anti-BrdU antibody. When cells are incubated in media containing BrdU, the pyrimidine analog is incorporated in place of thymidine into the newly synthesized DNA of proliferating cells. Once the labeling media is removed, the cells are fixed and the DNA is denatured in one step with a fix/denature solution (denaturation of the DNA is necessary to improve the accessibility of the incorporated BrdU for detection). Then an anti-BrdU mouse monoclonal antibody is added followed by an HRP conjugated secondary antibody to detect the incorporated BrdU. The magnitude of the absorbance for the developed color is proportional to the quantity of BrdU incorporated into cells and can be directly correlated to cell proliferation. |
CytoSelect 96-well Cell Transformation Assay |
CBA-130 |
Cell Biolabs |
96 assays |
EUR 866.4 |
Description: Our CytoSelect 96-Well Cell Transformation Assay (Soft Agar Colony Formation) is suitable for measuring cell transformation where no downstream analysis is required. Cells are incubated in a semisolid agar medium for 7-8 days. The cells are then solubilized, lysed and detected using the included fluorescent dye in a fluorometric plate reader. |
CytoSelect 96-well Cell Transformation Assay |
CBA-130-5 |
Cell Biolabs |
5 x 96 assays |
EUR 3463.2 |
Description: Our CytoSelect 96-Well Cell Transformation Assay (Soft Agar Colony Formation) is suitable for measuring cell transformation where no downstream analysis is required. Cells are incubated in a semisolid agar medium for 7-8 days. The cells are then solubilized, lysed and detected using the included fluorescent dye in a fluorometric plate reader. |
CytoSelect 24-well Cell Invasion, Fluorometric |
CBA-111 |
Cell Biolabs |
12 assays |
EUR 714 |
Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 24-well plates on either a standard microplate reader or a fluorescence plate reader. Inserts are precoated on the top of the membrane with ECM matrix gel (basement membrane), a protein mix isolated from EHS tumor cells. |
CytoSelect 96-well Cell Invasion, Fluorometric |
CBA-112 |
Cell Biolabs |
96 assays |
EUR 908.4 |
Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect 96-Well Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 96-well plates on a fluorescence plate reader. Inserts are precoated on the top of the membrane with Basement Membrane, an ECM protein mix isolated from EHS tumor cells. |
CytoSelect Cell Viability and Cytotoxicity Assay |
CBA-240 |
Cell Biolabs |
96 assays |
EUR 470.4 |
Description: The CytoSelect Cell Viability and Cytotoxicity Assay Kit provides a simple format for monitoring cell viability via metabolic activity. Live cells are detected with either MTT (colorimetric detection) or Calcein AM (fluorometric detection). Dead cells are detected by EthD-1 reagent (fluorometric). All 3 detection reagents are included, along with Saponin (a cell death initiator). Prior to the assay, cells may be treated with compounds or agents that affect cell viability. This kit is suitable for eukaryotic cells, not yeast or bacteria. |
CytoSelect 24-Well Cell Co-Culture System |
CBA-160 |
Cell Biolabs |
24 assays |
EUR 525.6 |
Description: CytoSelect 24-Well Cell Co-Culture System provides a unique platform to monitor direct contact between two cell types in a single well. First, cells are cultured until they form a monolayer around the insert, creating a defined 8 mm cell-free zone. Once the insert is removed, a second cell type may be seeded into the exposed zone. The kit contains proprietary treated inserts and sufficient reagents for the evaluation of 24 samples. The inserts are compatible with most adherent cell types and experimental conditions. |
CytoSelect 24-Well Cell Co-Culture System |
CBA-160-5 |
Cell Biolabs |
5 x 24 assays |
EUR 2078.4 |
Description: CytoSelect 24-Well Cell Co-Culture System provides a unique platform to monitor direct contact between two cell types in a single well. First, cells are cultured until they form a monolayer around the insert, creating a defined 8 mm cell-free zone. Once the insert is removed, a second cell type may be seeded into the exposed zone. The kit contains proprietary treated inserts and sufficient reagents for the evaluation of 24 samples. The inserts are compatible with most adherent cell types and experimental conditions. |
CytoSelect 48-Well Cell Contraction Assay Kit |
CBA-5021 |
Cell Biolabs |
48 assays |
EUR 762 |
Description: Cell Biolabs? Cell Contraction Assays (Floating Matrix Model) provide a simple, in vitro system to assess cell contractivity and screen cell contraction mediators. The proprietary Cell Contraction Plate eliminates the matrix releasing step of the conventional contraction assay, providing a faster, higher-throughput method to assess cell contraction. |
CytoSelect Cell Proliferation Assay Reagent
(Fluorometric) |
CBA-250 |
Cell Biolabs |
10 mL |
EUR 490.8 |
Description: Cell Biolabs? CytoSelect Cell Proliferation Assay Reagent (Fluorometric) provides a fluorometric format for measuring and monitoring cell proliferation. Cells can be plated and then treated with compounds or agents that affect proliferation. Cells are then incubated with the proliferation reagent. Upon entering metabolically active live cells, the non-fluorescent proliferation reagent is converted into a bright red fluorescent form. An increase in cell proliferation is accompanied by increased fluorescent signal, while a decrease in cell proliferation (and signal) can indicate the toxic effects of compounds or suboptimal culture conditions. The assay principles are basic and can be applied to most eukaryotic cell lines, including adherent and non-adherent cells and certain tissues. This cell proliferation reagent can be used to detect proliferation in bacteria, yeast, fungi, protozoa as well as cultured mammalian and piscine cells. The kit contains sufficient reagents for the evaluation of 960 assays in ten 96-well plates or 192 assays in eight 24-well plates. |
CytoSelect Cell Proliferation Assay Reagent (Colorimetric) |
CBA-253 |
Cell Biolabs |
10 mL |
EUR 490.8 |
Description: Cell Biolabs? CytoSelect WST-1 Cell Proliferation Assay Reagent provides a colorimetric format for measuring and monitoring cell proliferation. The 10 mL volume is sufficient for the evaluation of 960 assays in ten 96-well plates or 192 assays in eight 24-well plates. Cells can be plated and then treated with compounds or agents that affect proliferation. Cells are then detected with the proliferation reagent, which is converted in live cells from WST-1 to the formazan form in the presence of cellular NADH and an electron mediator. An increase in cell proliferation is accompanied by increased signal, while a decrease in cell proliferation (and signal) can indicate the toxic effects of compounds or suboptimal culture conditions. The assay principles are basic and can be applied to most eukaryotic cell lines, including adherent and non-adherent cells and certain tissues. This cell proliferation reagent can be used to detect proliferation in bacteria, yeast, fungi, protozoa as well as cultured mammalian and piscine cells. |
CytoSelect 24-well Cell Invasion Assay, Colorimetric |
CBA-110 |
Cell Biolabs |
12 assays |
EUR 714 |
Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 24-well plates on either a standard microplate reader or a fluorescence plate reader. Inserts are precoated on the top of the membrane with ECM matrix gel (basement membrane), a protein mix isolated from EHS tumor cells. |
CytoSelect 24-well Laminin Cell Invasion, Colorimetric |
CBA-110-LN |
Cell Biolabs |
12 assays |
EUR 714 |
Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 24-well plates on either a standard microplate reader or a fluorescence plate reader. Inserts are precoated on the top of the membrane with Laminin. |
Radius 24-Well Cell Migration Assay, (Laminin Coated) |
CBA-125-LN |
Cell Biolabs |
24 assays |
EUR 714 |
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap. |
CytoSelect 24-well Collagen Cell Invasion, Colorimetric |
CBA-110-COL |
Cell Biolabs |
12 assays |
EUR 714 |
Description: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the morbidity and mortality of cancers. Cell invasion requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Our CytoSelect Cell Invasion Assays utilize precoated inserts to assay the invasive properties of tumor cells. Invasive cells can be quantified in 24-well plates on either a standard microplate reader or a fluorescence plate reader. Inserts are precoated on the top of the membrane with Collagen I. |
StemTAG PCR Primer Set for Stem Cell Characterization |
CBA-303 |
Cell Biolabs |
1 kit |
EUR 470.4 |
Description: StemTAG PCR Primer Set for Stem Cell Characterization includes 7 primer pairs: Oct-4, NANOG, AFP, Flk-1, and NCAM, plus GAPDH and beta-actin as controls. |
CytoSelect 96-well Phagocytosis Assay (Red Blood Cell) |
CBA-220 |
Cell Biolabs |
96 assays |
EUR 762 |
Description: Phagocytosis can be assayed by measuring the engulfment of a cell "substrate". However, traditional assays require tedious cell counting under a microscope. Our CytoSelect 96-Well Phagocytosis Assay, Red Blood Cell Substrate provides a more accurate, user-friendly, high-throughput alternative to the standard phagocytosis assay. The assay may be adapted for use with 24-well or 48-well plates. |
CytoSelect Clonogenic Tumor Cell Isolation Kit (5 preps) |
CBA-155 |
Cell Biolabs |
5 preps |
EUR 957.6 |
Description: Many solid tumors contain heterogeneous populations of normal and cancerous cells. Separation of these cell populations is key to an accurate assessment of the true genotypic and phenotypic differences between normal and tumor cells. Our CytoSelect Clonogenic Tumor Cell Isolation Kit uses a proprietary semisolid agar medium to facilitate formation of colonies by cells from solid tumors. Colonies are grown in either a 6-well plate or a 35mm culture dish. These colonies are isolated away from single (i.e. normal) cells by size filtration. The viable cells from these colonies can be easily recovered for further analysis. |
CytoSelect 384-well Cell Transformation Assay, Fluorometric |
CBA-145 |
Cell Biolabs |
384 assays |
EUR 1208.4 |
Description: Our CytoSelect 384-Well Cell Transformation Assay uses a modified soft agar 3D matrix to support the formation of colonies by neoplastic cells. Quantitation of cell transformation is performed on a fluorescence plate reader. |
CytoSelect 384-well Cell Transformation Assay, Fluorometric |
CBA-145-5 |
Cell Biolabs |
5 x 384 assays |
EUR 4681.2 |
Description: Our CytoSelect 384-Well Cell Transformation Assay uses a modified soft agar 3D matrix to support the formation of colonies by neoplastic cells. Quantitation of cell transformation is performed on a fluorescence plate reader. |
Radius 24-Well Cell Migration Assay, (Fibronectin Coated) |
CBA-125-FN |
Cell Biolabs |
24 assays |
EUR 714 |
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap. |
Radius 24-Well Cell Migration Assay, (ECM Array Coated) |
CBA-125-ECM |
Cell Biolabs |
24 wells |
EUR 838.8 |
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap. |
Radius 24-Well Cell Migration Assay, (Collagen I Coated) |
CBA-125-COL |
Cell Biolabs |
24 assays |
EUR 714 |
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap. |
CytoSelect Proliferating Cell Nuclear Antigen (PCNA) ELISA Kit |
CBA-254 |
Cell Biolabs |
96 assays |
EUR 672 |
Description: Cell Biolabs? CytoSelect Proliferating Cell Nuclear Antigen (PCNA) ELISA Kit is an enzyme immunoassay developed for the detection and quantitation of PCNA from nuclear and whole cell extracts. The kit detects PCNA from mouse, rat and human, and has a detection sensitivity limit of 12.5 ng/mLPCNA. Each kit provides sufficient reagents to perform up to 96 assays including standard curve and unknown samples. |
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Sufferers within the nHM group had the next danger of pheochromocytoma (HR = 3.447, p < 0.001) and decrease dangers of central nervous system hemangioblastoma (CHB) (HR = 0.700, p = 0.045), renal cell carcinoma (HR = 0.610, p = 0.024), and pancreatic tumor (HR = 0.382, p < 0.001) than these within the mixed HM and TR (HMTR) group.
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Furthermore, nHM mutations had been independently related to better general survival (HR = 0.345, p = 0.005) and CHB-specific survival (HR = 0.129, p = 0.005) than HMTR mutations.
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The modified genotype-phenotype correlation hyperlinks VHL gene mutation, substrate binding web site, and phenotypic range (penetrance and survival), and offers extra correct data for genetic counseling and pathogenesis research.