Von Hippel-Lindau (VHL) Protein Antagonist VH298 Improves Wound Therapeutic in Streptozotocin-Induced Hyperglycaemic Rats by Activating Hypoxia-Inducible Issue- (HIF-) 1 Signalling.

 

The aim of the current analysis is to analyze the results of the VHL protein antagonist, VH298, on purposeful actions of fibroblasts and vascular endothelial cells and the results on the wound therapeutic course of in a streptozotocin-induced hyperglycaemic rat mannequin.

HIF-1α and hydroxy-HIF-1α protein ranges in VH298-treated rat fibroblasts (rFb) had been measured by immunoblotting, rFb proliferation was detected by the CCK-Eight assay, and mRNA ranges of associated genes had been measured by quantitative RT-PCR. In vitro wound therapeutic was simulated by the scratch take a look at; angiogenesis was measured by the human umbilical vein endothelial cell (hUVEC) tube formation assay. VH298 or PBS was regionally injected into wounds in rat fashions with streptozotocbd tissues had been harvested, and haematoxylin-eosin (HE) and Masson trichrome staining and immunohistochemical processes had been carried out.

Human Von Hippel Lindau Tumor Suppressor (vHL) ELISA Equipment

HIF-1α and hydroxy-HIF-1α ranges elevated in VH298-treated rFb, in a time- and dose-dependent method. Thirty micromolar VH298 may considerably enhance cell proliferation, angiogenesis, and gene expression of sort I collagen-αα

Taken collectively, VH298 activated the HIF-1 signalling pathway by stabilizing each HIF-1α and hydroxy-HIF-1α. VH298 enhanced rFb capabilities, promoted hUVEC angiogenesis, and accelerated wound therapeutic within the rat mannequin mimicking diabetes mellitus.

Rat Von Hippel Lindau Tumor Suppressor (vHL) ELISA Equipment

 

 

Human Von Hippel Lindau Tumor Suppressor (vHL) ELISA Kit
RD-vHL-Hu-48Tests	Reddot Biotech	48 Tests	EUR 557

Human Von Hippel Lindau Tumor Suppressor (vHL) ELISA Kit
RD-vHL-Hu-96Tests	Reddot Biotech	96 Tests	EUR 775

Rat Von Hippel Lindau Tumor Suppressor (vHL) ELISA Kit
RD-vHL-Ra-48Tests	Reddot Biotech	48 Tests	EUR 557

Rat Von Hippel Lindau Tumor Suppressor (vHL) ELISA Kit
RD-vHL-Ra-96Tests	Reddot Biotech	96 Tests	EUR 775

Human Von Hippel Lindau Tumor Suppressor (vHL) ELISA Kit
RDR-vHL-Hu-48Tests	Reddot Biotech	48 Tests	EUR 583

Human Von Hippel Lindau Tumor Suppressor (vHL) ELISA Kit
RDR-vHL-Hu-96Tests	Reddot Biotech	96 Tests	EUR 811

Rat Von Hippel Lindau Tumor Suppressor (vHL) ELISA Kit
RDR-vHL-Ra-48Tests	Reddot Biotech	48 Tests	EUR 583

Rat Von Hippel Lindau Tumor Suppressor (vHL) ELISA Kit
RDR-vHL-Ra-96Tests	Reddot Biotech	96 Tests	EUR 811

VHL antibody
70R-9757	Fitzgerald	50 ug	EUR 467
Description: Affinity purified rabbit polyclonal VHL antibody

VHL antibody
70R-34605	Fitzgerald	100 ug	EUR 327
Description: Purified Rabbit polyclonal VHL antibody

VHL Antibody
AF6292	Affbiotech	200ul	EUR 304
Description: VHL Antibody detects endogenous levels of total VHL.

VHL Antibody
ABF6292	Lifescience Market	100 ug	EUR 438

VHL Antibody
ABD6104	Lifescience Market	100 ug	EUR 438

VHL antibody
10R-1029	Fitzgerald	100 ul	EUR 316
Description: Mouse monoclonal VHL antibody

VHL Antibody
DF6104	Affbiotech	200ul	EUR 304
Description: VHL Antibody detects endogenous levels of total VHL.

VHL Antibody
1-CSB-PA060067	Cusabio	EUR 222.00 EUR 195.00	100ug 50ug
Description: A polyclonal antibody against VHL. Recognizes VHL from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: IHC, ELISA;IHC:1/100-1/300.ELISA:1/40000

VHL Antibody
1-CSB-PA071125	Cusabio	EUR 317.00 EUR 244.00	100ul 50ul
Description: A polyclonal antibody against VHL. Recognizes VHL from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:2000-1:5000, WB:1:500-1:2000, IHC:1:25-1:100

VHL Antibody
CSB-PA025852KA01HU-	Cusabio		EUR 335
Description: A polyclonal antibody against VHL. Recognizes VHL from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:2000, IHC:1:50-1:200

VHL Antibody
CSB-PA025852KA01HU-100ul	Cusabio	100ul	EUR 389
Description: A polyclonal antibody against VHL. Recognizes VHL from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:2000, IHC:1:50-1:200

VHL Antibody
1-CSB-PA876839	Cusabio	EUR 222.00 EUR 195.00	100ug 50ug
Description: A polyclonal antibody against VHL. Recognizes VHL from Human. This antibody is Unconjugated. Tested in the following application: IHC, ELISA;IHC-p:1:50-300, ELISA:1:10000-20000

VHL Antibody
1-CSB-PA991849	Cusabio	EUR 317.00 EUR 244.00	100ul 50ul
Description: A polyclonal antibody against VHL. Recognizes VHL from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:2000-1:10000, IHC:1:50-1:200

VHL Antibody
32075-100ul	SAB	100ul	EUR 252

VHL/ Rat VHL ELISA Kit
ELA-E1894r	Lifescience Market	96 Tests	EUR 886

Vhl/ Rat Vhl ELISA Kit
ELI-06080r	Lifescience Market	96 Tests	EUR 886

Anti-VHL antibody
PAab09402	Lifescience Market	100 ug	EUR 386

VHL Polyclonal Antibody
ES7499-100ul	ELK Biotech	100ul	EUR 279
Description: A Rabbit Polyclonal antibody against VHL from Human/Mouse/Rat. This antibody is tested and validated for IHC, WB, ELISA

VHL Polyclonal Antibody
ES7499-50ul	ELK Biotech	50ul	EUR 207
Description: A Rabbit Polyclonal antibody against VHL from Human/Mouse/Rat. This antibody is tested and validated for IHC, WB, ELISA

VHL Polyclonal Antibody
ES8746-100ul	ELK Biotech	100ul	EUR 279
Description: A Rabbit Polyclonal antibody against VHL from Human. This antibody is tested and validated for IHC, WB, ELISA

VHL Polyclonal Antibody
ES8746-50ul	ELK Biotech	50ul	EUR 207
Description: A Rabbit Polyclonal antibody against VHL from Human. This antibody is tested and validated for IHC, WB, ELISA

VHL Polyclonal Antibody
ABP60891-003ml	Abbkine	0.03ml	EUR 158
Description: A polyclonal antibody for detection of VHL from Human. This VHL antibody is for IHC-P, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human VHL protein at amino acid sequence of 1-50

VHL Polyclonal Antibody
ABP60891-01ml	Abbkine	0.1ml	EUR 289
Description: A polyclonal antibody for detection of VHL from Human. This VHL antibody is for IHC-P, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human VHL protein at amino acid sequence of 1-50

VHL Polyclonal Antibody
ABP60891-02ml	Abbkine	0.2ml	EUR 414
Description: A polyclonal antibody for detection of VHL from Human. This VHL antibody is for IHC-P, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human VHL protein at amino acid sequence of 1-50

VHL Polyclonal Antibody
ABP56500-003ml	Abbkine	0.03ml	EUR 158
Description: A polyclonal antibody for detection of VHL from Human, Mouse, Rat. This VHL antibody is for IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human VHL around the non-phosphorylation site of S68

VHL Polyclonal Antibody
ABP56500-01ml	Abbkine	0.1ml	EUR 289
Description: A polyclonal antibody for detection of VHL from Human, Mouse, Rat. This VHL antibody is for IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human VHL around the non-phosphorylation site of S68

VHL Polyclonal Antibody
ABP56500-02ml	Abbkine	0.2ml	EUR 414
Description: A polyclonal antibody for detection of VHL from Human, Mouse, Rat. This VHL antibody is for IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human VHL around the non-phosphorylation site of S68

VHL (pS68) Antibody
abx219320-100ug	Abbexa	100 ug	EUR 439

Anti-VHL antibody
STJ26089	St John's Laboratory	100 µl	EUR 277
Description: Von Hippel-Lindau syndrome (VHL) is a dominantly inherited familial cancer syndrome predisposing to a variety of malignant and benign tumors. A germline mutation of this gene is the basis of familial inheritance of VHL syndrome. The protein encoded by this gene is a component of the protein complex that includes elongin B, elongin C, and cullin-2, and possesses ubiquitin ligase E3 activity. This protein is involved in the ubiquitination and degradation of hypoxia-inducible-factor (HIF), which is a transcription factor that plays a central role in the regulation of gene expression by oxygen. RNA polymerase II subunit POLR2G/RPB7 is also reported to be a target of this protein. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Anti-VHL antibody
STJ98809	St John's Laboratory	200 µl	EUR 197
Description: Rabbit polyclonal to VHL.

Anti-VHL antibody
STJ96241	St John's Laboratory	200 µl	EUR 197
Description: Rabbit polyclonal to VHL.

Anti-VHL Antibody
STJ502735	St John's Laboratory	100 µg	EUR 476

Anti-VHL antibody
STJ70964	St John's Laboratory	100 µg	EUR 359

Human VHL Antibody
32825-05111	AssayPro	150 ug	EUR 261

Anti-VHL antibody
STJ118733	St John's Laboratory	100 µl	EUR 277

Anti-VHL antibody
STJ113440	St John's Laboratory	50 µl	EUR 277
Description: Von Hippel-Lindau syndrome (VHL) is a dominantly inherited familial cancer syndrome predisposing to a variety of malignant and benign tumors. A germline mutation of this gene is the basis of familial inheritance of VHL syndrome. The protein encoded by this gene is a component of the protein complex that includes elongin B, elongin C, and cullin-2, and possesses ubiquitin ligase E3 activity. This protein is involved in the ubiquitination and degradation of hypoxia-inducible-factor (HIF), which is a transcription factor that plays a central role in the regulation of gene expression by oxygen. RNA polymerase II subunit POLR2G/RPB7 is also reported to be a target of this protein. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Anti-VHL antibody
STJ113742	St John's Laboratory	100 µl	EUR 277
Description: Von Hippel-Lindau syndrome (VHL) is a dominantly inherited familial cancer syndrome predisposing to a variety of malignant and benign tumors. A germline mutation of this gene is the basis of familial inheritance of VHL syndrome. The protein encoded by this gene is a component of the protein complex that includes elongin B, elongin C, and cullin-2, and possesses ubiquitin ligase E3 activity. This protein is involved in the ubiquitination and degradation of hypoxia-inducible-factor (HIF), which is a transcription factor that plays a central role in the regulation of gene expression by oxygen. RNA polymerase II subunit POLR2G/RPB7 is also reported to be a target of this protein. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

VHL Polyclonal Antibody
46866-100ul	SAB	100ul	EUR 252

VHL Polyclonal Antibody
46866-50ul	SAB	50ul	EUR 187

VHL Conjugated Antibody
C32075	SAB	100ul	EUR 397

anti- VHL antibody
FNab10175	FN Test	100µg	EUR 505.25
Description: Antibody raised against VHL

 

 

VHL Dependent Expression of REDD1 and PDK3 Proteins in Clear-cell Renal Cell Carcinoma.

 

 

• Sporadic clear-cell renal cell carcinoma (ccRCC) is related with mutations within the VHL gene, upregulated mammalian goal of rapamycin (mTOR) exercise and glycolytic metabolism. Right here, we analyze the impact of VHL mutational standing on the expression stage of mTOR, eIF4E-BP1, AMPK, REDD1, and PDK3 proteins.

pGF-AP1-mCMV-EF1-Puro (293 secure cell line)

• Complete proteins had been remoted from 21 tumorous samples with biallelic inactivation, 10 with monoallelic inactivation and 6 tumors with a wild-type VHL (wtVHL) gene obtained from sufferers who underwent complete nephrectomy. The expressions of goal proteins had been assessed utilizing Western blot.Expressions of mTOR, eIF4EBP1 and AMPOkay had been VHL impartial.

 

• Tumors with monoallelic inactivation of VHL underexpressed REDD1 compared to wtVHL tumors (P = 0.042), tumors with biallelic VHL inactivation (P < 0.005) and management tissue (P = 0.004). Moreover, REDD1 expression was larger in tumors with VHL biallelic inactivation than in management tissue (P = 0.008). Solely in wt tumor samples PDK3 was overexpressed compared to tumors with biallelic inactivation of VHL gene (P = 0.012) and controls (P = 0.016). In wtVHL ccRCC, multivariate linear regression evaluation revealed that 97.4% of variability in PDK3 expression might be defined by variations in AMPK quantity.Expressions of mTOR, eIF4EBP1 and AMPK had been VHL impartial.

 

• Now we have proven for the primary time VHL dependent expression of PDK3 and we offer extra proof that VHL mutational standing impacts REDD1 expression in sporadic ccRCC.

Gene Knock-Out HR Concentrating on Vector w/Single Choice Marker (Blasticidin) and Destructive Choice (TK) Towards Random Integration

• Von Hippel-Lindau (VHL) illness is a uncommon hereditary most cancers syndrome that reduces life expectancy. We aimed to assemble a extra useful genotype-phenotype correlation primarily based on alterations in VHL protein (pVHL).

 

• VHL sufferers (n = 339) had been recruited and grouped primarily based on mutation sorts: HIF-α binding web site missense (HM) mutations, non-HIF-α binding web site missense (nHM) mutations, and truncating (TR) mutations. Age-related dangers of VHL-associated tumors and affected person survival had been in contrast.

 

• Missense mutations conferred an elevated danger of pheochromocytoma (HR = 1.854, p = 0.047) in contrast with truncating mutations. The danger of pheochromocytoma was decrease within the HM group than within the nHM group (HR = 0.298, p = 0.003) however was related between HM and TR teams (HR = 0.901, p = 0.810).

Human Von Hippel Lindau Tumor Suppressor (vHL) ELISA Kit
RDR-vHL-Hu-48Tests	Reddot Biotech	48 Tests	EUR 583

Human Von Hippel Lindau Tumor Suppressor (vHL) ELISA Kit
RDR-vHL-Hu-96Tests	Reddot Biotech	96 Tests	EUR 811

Gene Knock-Out HR Targeting Vector [MCS1-EF1?-RFP-T2A-Puro-pA-MCS2]
HR110PA-1	SBI	10 ug	EUR 1023

Gene Knock-Out HR Targeting Vector [MCS1-EF1a-GFP-T2A-Puro-pA-MCS2]
HR410PA-1	SBI	10 ug	EUR 1023

Gene Knock-Out HR Targeting Vector [MCS1-EF1a-RFP-T2A-Hygro-pA-MCS2]
HR510PA-1	SBI	10 ug	EUR 1023

TARGATT? Knock-in iPSC Generation (Master Cell Line)
AST-1100	Applied StemCell	1 vial of 1X10^6 cells	Ask for price
Description: 6 month

TARGATT? Knock-in Mouse Cell Line Generation Kit (Master Cell Line)
AST-7001	Applied StemCell	1 Kit	Ask for price
Description: 6 month

Rat Von Hippel Lindau Tumor Suppressor (vHL) ELISA Kit
DLR-vHL-Ra-48T	DL Develop	48T	EUR 549
Description: A sandwich quantitative ELISA assay kit for detection of Rat Von Hippel Lindau Tumor Suppressor (vHL) in samples from tissue homogenates or other biological fluids.

Rat Von Hippel Lindau Tumor Suppressor (vHL) ELISA Kit
DLR-vHL-Ra-96T	DL Develop	96T	EUR 718
Description: A sandwich quantitative ELISA assay kit for detection of Rat Von Hippel Lindau Tumor Suppressor (vHL) in samples from tissue homogenates or other biological fluids.

Rat Von Hippel Lindau Tumor Suppressor (vHL) ELISA Kit
RD-vHL-Ra-48Tests	Reddot Biotech	48 Tests	EUR 557

Rat Von Hippel Lindau Tumor Suppressor (vHL) ELISA Kit
RD-vHL-Ra-96Tests	Reddot Biotech	96 Tests	EUR 775

Rat Von Hippel Lindau Tumor Suppressor (vHL) ELISA Kit
RDR-vHL-Ra-48Tests	Reddot Biotech	48 Tests	EUR 583

Rat Von Hippel Lindau Tumor Suppressor (vHL) ELISA Kit
RDR-vHL-Ra-96Tests	Reddot Biotech	96 Tests	EUR 811

Vhl 3'UTR Luciferase Stable Cell Line
TU121762	ABM	1.0 ml	Ask for price

VHL 3'UTR GFP Stable Cell Line
TU078117	ABM	1.0 ml	EUR 1521

Vhl 3'UTR GFP Stable Cell Line
TU171762	ABM	1.0 ml	Ask for price

Vhl 3'UTR Luciferase Stable Cell Line
TU223038	ABM	1.0 ml	Ask for price

VHL 3'UTR Luciferase Stable Cell Line
TU028117	ABM	1.0 ml	EUR 1521

Vhl 3'UTR GFP Stable Cell Line
TU273038	ABM	1.0 ml	Ask for price

TARGATT? Knock-in CHO Generation Kit (Master Cell Line)
AST-1200	Applied StemCell	1 Kit	Ask for price
Description: 12 month

TARGATT? Knock-in HEK293 Generation Kit (Master Cell Line)
AST-1300	Applied StemCell	1 Kit	Ask for price
Description: 12 month

293LTV Cell Line
LTV-100	Cell Biolabs	1 vial	EUR 508
Description: The 293LTV Cell Line is derived from the parental 293 cells but selected for attributes that increase lentiviral production, including fimrer attachment and larger surface area.

293AD Cell Line
AD-100	Cell Biolabs	1 vial	EUR 461
Description: The 293AD Cell Line is derived from the parental 293 cells but selected for attributes that increase adenovirus production, including firmer attachment and larger surface area.

293AAV Cell Line
AAV-100	Cell Biolabs	1 vial	EUR 508
Description: The 293AAV Cell Line is derived from the parental 293 cells but selected for attributes that increase AAV production, including firmer attachment and larger surface area.

293RTV Cell Line
RV-100	Cell Biolabs	1 vial	EUR 508
Description: The 293RTV Cell Line is derived from the parental 293 cells but selected for attributes that increase retroviral production, including fimrer attachment and larger surface area.

Basic HR Targeting Vector [MCS1-LoxP-MCS2-MCS3-pA-LoxP-MCS4] for Gene Knock-In/Out
HR100PA-1	SBI	10 ug	EUR 938

T47D/GFP Cell Line
AKR-208	Cell Biolabs	1 vial	EUR 572
Description: T47D/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

A549/GFP Cell Line
AKR-209	Cell Biolabs	1 vial	EUR 572
Description: A549/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

HeLa/GFP Cell Line
AKR-213	Cell Biolabs	1 vial	EUR 572
Description: HeLa/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

NIH3T3/GFP Cell Line
AKR-214	Cell Biolabs	1 vial	EUR 572
Description: NIH3T3/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

NIH3T3/Cas9 Cell Line
AKR-5104	Cell Biolabs	1 vial	EUR 572

HeLa/Cas9 Cell Line
AKR-5111	Cell Biolabs	1 vial	EUR 572

VHL/ Rat VHL ELISA Kit
ELA-E1894r	Lifescience Market	96 Tests	EUR 886

Vhl/ Rat Vhl ELISA Kit
ELI-06080r	Lifescience Market	96 Tests	EUR 886

Human VHL Antibody
32825-05111	AssayPro	150 ug	EUR 261

Recombinant human VHL
P2120	FN Test	100ug	Ask for price
Description: Recombinant protein for human VHL

Mouse pre-microRNA Expression Construct mir-293+294+295
MMIR-293-295-PA-CL	SBI	Bacterial Streak	EUR 968

Microplate Swing-Out Centrifuge
abx725026-1Unit	Abbexa	1 Unit	EUR 1476

Clinical Swing-Out Centrifuge
abx725027-1Unit	Abbexa	1 Unit	EUR 1476

SKOV-3/Luc Cell Line
AKR-232	Cell Biolabs	1 vial	EUR 572
Description: SKOV-3/Luc Cell Line stably expresses luciferase and otherwise exhibits the same characteristics of the parental cell line.

MCF-7/Luc Cell Line
AKR-234	Cell Biolabs	1 vial	EUR 572
Description: MCF-7/Luc Cell Line stably expresses luciferase and otherwise exhibits the same characteristics of the parental cell line.

OVCAR-5/RFP Cell Line
AKR-254	Cell Biolabs	1 vial	EUR 572
Description: OVCAR-5/RFP Cell Line stably expresses RFP and otherwise exhibits the same characteristics of the parental cell line.

VHL sgRNA CRISPR Lentivector set (Human)
K2611301	ABM	3 x 1.0 ug	EUR 339

Gene Knock-Out HR Targeting Vector w/Single Selection Marker (Blasticidin) and Negative Selection (TK) Against Random Integration
HR720PA-1	SBI	10 µg	EUR 1145

VHL antibody
70R-9757	Fitzgerald	50 ug	EUR 467
Description: Affinity purified rabbit polyclonal VHL antibody

VHL antibody
70R-34605	Fitzgerald	100 ug	EUR 327
Description: Purified Rabbit polyclonal VHL antibody

VHL Antibody
AF6292	Affbiotech	200ul	EUR 304
Description: VHL Antibody detects endogenous levels of total VHL.

VHL Antibody
ABF6292	Lifescience Market	100 ug	EUR 438

VHL Antibody
ABD6104	Lifescience Market	100 ug	EUR 438

VHL antibody
10R-1029	Fitzgerald	100 ul	EUR 316
Description: Mouse monoclonal VHL antibody

VHL Antibody
DF6104	Affbiotech	200ul	EUR 304
Description: VHL Antibody detects endogenous levels of total VHL.

VHL Antibody
1-CSB-PA060067	Cusabio	EUR 222.00 EUR 195.00	100ug 50ug
Description: A polyclonal antibody against VHL. Recognizes VHL from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: IHC, ELISA;IHC:1/100-1/300.ELISA:1/40000

VHL Antibody
1-CSB-PA071125	Cusabio	EUR 317.00 EUR 244.00	100ul 50ul
Description: A polyclonal antibody against VHL. Recognizes VHL from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:2000-1:5000, WB:1:500-1:2000, IHC:1:25-1:100

VHL Antibody
CSB-PA025852KA01HU-	Cusabio		EUR 335
Description: A polyclonal antibody against VHL. Recognizes VHL from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:2000, IHC:1:50-1:200

VHL Antibody
CSB-PA025852KA01HU-100ul	Cusabio	100ul	EUR 389
Description: A polyclonal antibody against VHL. Recognizes VHL from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:2000, IHC:1:50-1:200

VHL Antibody
1-CSB-PA876839	Cusabio	EUR 222.00 EUR 195.00	100ug 50ug
Description: A polyclonal antibody against VHL. Recognizes VHL from Human. This antibody is Unconjugated. Tested in the following application: IHC, ELISA;IHC-p:1:50-300, ELISA:1:10000-20000

VHL Antibody
1-CSB-PA991849	Cusabio	EUR 317.00 EUR 244.00	100ul 50ul
Description: A polyclonal antibody against VHL. Recognizes VHL from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:2000-1:10000, IHC:1:50-1:200

VHL siRNA
20-abx939411	Abbexa	EUR 551.00 EUR 732.00	15 nmol 30 nmol

VHL siRNA
20-abx939412	Abbexa	EUR 551.00 EUR 732.00	15 nmol 30 nmol

VHL Antibody
32075-100ul	SAB	100ul	EUR 252

VHL siRNA
20-abx906024	Abbexa	EUR 551.00 EUR 732.00	15 nmol 30 nmol

pGF-AP1-mCMV-EF1-Puro (293 stable cell line)
TR201a-p	SBI	>2 x 10^6 cells	EUR 3263

pGF-CREB-mCMV-EF1-Puro (293 stable cell line)
TR202a-p	SBI	>2 x 10^6 cells	EUR 3263

pGF-EGR1-mCMV-EF1-Puro (293 stable cell line)
TR204a-p	SBI	>2 x 10^6 cells	EUR 3263

NF-kB/293/GFP-Luc Transcriptional Reporter Cell Line
TR860A-1	SBI	>2 x 10^6 cells	EUR 3263

Human VHL ELISA Kit
ELA-E1894h	Lifescience Market	96 Tests	EUR 824

VHL ELISA KIT|Human
EF006088	Lifescience Market	96 Tests	EUR 689

VHL Recombinant Protein (Human)
RP034324	ABM	100 ug	Ask for price

Human VHL shRNA Plasmid
20-abx955082	Abbexa	EUR 801.00 EUR 1121.00	150 µg 300 µg

Gene Knock-Out HR Targeting Vector w/Dual Selection Markers (GFP+Puro) and Negative Selection (TK) Against Random Integration
HR700PA-1	SBI	10 µg	EUR 1145

Gene Knock-Out HR Targeting Vector w/Dual Selection Markers (RFP+Hygro) and Negative Selection (TK) Against Random Integration
HR710PA-1	SBI	10 µg	EUR 1145

Platinum-E Retroviral Packaging Cell Line, Ecotropic
RV-101	Cell Biolabs	1 vial	EUR 920
Description: Conventional cells used for retrovirus packaging, such as those based on NIH3T3 cells, have limited stability and produce relatively low yields of retrovirus, mainly due to the poor expression of retroviral structure proteins (gag, pol and env) in the cells. The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line. They exhibit longer stability and produce higher yields of retroviral structure proteins. Plat-E cells contain gag, pol and env genes, allowing retroviral packaging with a single plasmid transfection.

Platinum-A Retroviral Packaging Cell Line, Amphotropic
RV-102	Cell Biolabs	1 vial	EUR 920
Description: Conventional cells used for retrovirus packaging, such as those based on NIH3T3 cells, have limited stability and produce relatively low yields of retrovirus, mainly due to the poor expression of retroviral structure proteins (gag, pol and env) in the cells. The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line. They exhibit longer stability and produce higher yields of retroviral structure proteins. Plat-A cells contain gag, pol and env genes, allowing retroviral packaging with a single plasmid transfection.

Platinum-GP Retroviral Packaging Cell Line, Pantropic
RV-103	Cell Biolabs	1 vial	EUR 920
Description: Conventional cells used for retrovirus packaging, such as those based on NIH3T3 cells, have limited stability and produce relatively low yields of retrovirus, mainly due to the poor expression of retroviral structure proteins (gag, pol and env) in the cells. The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line. They exhibit longer stability and produce higher yields of retroviral structure proteins. Plat-GP cells contain the gag and pol genes required for retroviral packaging; an expression vector is co-transfected with a VSVG envelope vector.

VHL sgRNA CRISPR Lentivector (Human) (Target 1)
K2611302	ABM	1.0 ug DNA	EUR 154

VHL sgRNA CRISPR Lentivector (Human) (Target 2)
K2611303	ABM	1.0 ug DNA	EUR 154

VHL sgRNA CRISPR Lentivector (Human) (Target 3)
K2611304	ABM	1.0 ug DNA	EUR 154

PIK-293
B1658-25	Biovision		EUR 800

PIK-293
B1658-5	Biovision		EUR 240

BAY-293
HY-114398	MedChemExpress	50mg	EUR 1955

PIK-293
E1KS2207	EnoGene	5mg	EUR 599

PIK-293
B2187-10	ApexBio	10 mg	EUR 440
Description: PIK-293 is a PI3K inhibitor, mostly for PI3K? with IC50 of 0.24 ?M, 500-, 100- and 50-fold less potent to PI3K?/?/?, respectively.

PIK-293
B2187-25	ApexBio	25 mg	EUR 902
Description: PIK-293 is a PI3K inhibitor, mostly for PI3K? with IC50 of 0.24 ?M, 500-, 100- and 50-fold less potent to PI3K?/?/?, respectively.

PIK-293
B2187-5	ApexBio	5 mg	EUR 270
Description: PIK-293 is a PI3K inhibitor, mostly for PI3K? with IC50 of 0.24 ?M, 500-, 100- and 50-fold less potent to PI3K?/?/?, respectively.

IBC 293
B7069-10	ApexBio	10 mg	EUR 197

IBC 293
B7069-5.1	ApexBio	10 mM (in 1mL DMSO)	EUR 108

IBC 293
B7069-50	ApexBio	50 mg	EUR 669

Total Protein - Murine Embryonic Stem Cell Line D3
CBA-305	Cell Biolabs	500 ?g	EUR 345
Description: Isolated from mouse ES-D3 cell line Presented as 500 µg at 1 mg/mL in NP-40 Solubilization Buffer

Gene Knock-Out HR Targeting Vector with TK selection [MCS1-LoxP-EF1?-GFP-T2A-Puro-P2A-hsvTK-pA-LoxP-MCS2]
HR210PA-1	SBI	10 ug	EUR 1145

VHL Blocking Peptide
AF6292-BP	Affbiotech	1mg	EUR 195

• Sufferers within the nHM group had the next danger of pheochromocytoma (HR = 3.447, p < 0.001) and decrease dangers of central nervous system hemangioblastoma (CHB) (HR = 0.700, p = 0.045), renal cell carcinoma (HR = 0.610, p = 0.024), and pancreatic tumor (HR = 0.382, p < 0.001) than these within the mixed HM and TR (HMTR) group.

 

• Furthermore, nHM mutations had been independently related to better general survival (HR = 0.345, p = 0.005) and CHB-specific survival (HR = 0.129, p = 0.005) than HMTR mutations.

 

• The modified genotype-phenotype correlation hyperlinks VHL gene mutation, substrate binding web site, and phenotypic range (penetrance and survival), and offers extra correct data for genetic counseling and pathogenesis research.